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Journal: Microcirculation (New York, N.Y. : 1994)
Article Title: K IR channel regulation of electrical conduction along cerebrovascular endothelium: Enhanced modulation during Alzheimer’s disease
doi: 10.1111/micc.12797
Figure Lengend Snippet: Presence of connexins and inward-rectifying K+ (KIR) channels in cerebrovascular endothelium of young and aged 3xTg-AD mice. Endothelial tube immunofluorescence: (A) Immunofluorescent image of endothelial Cx37 in a young, Pre-AD mouse (1 to 2 mo). (B) As in A for Cx40. (C) As in B for KIR2.1. (D, E, F) As in A, B, and C respectively for aged, AD mice (14 to 16 mo). Images represent n=3 isolated cerebral endothelial tubes of 3 different animals (1 male & 2 females or 2 males & 1 female) per group. Scale bar = 50 μm.
Article Snippet: The endothelial tube was incubated overnight at 4°C with one of the following primary antibodies:
Techniques: Immunofluorescence, Isolation